Fig. 2.
ES induced neuronal hyperexcitability in rat TG neurons a, Representative images of TG neurons stained with IB4 in the sham and ES-treated groups. TG neurons were labelled with 3 μg/ml Alexa Fluor 594-conjugated IB4 for 10 min after the recordings. Scale bar = 50 μm. b and c, Exemple traces of action potential firing (b) and summary data (c) indicating that ES significantly increased the firing frequency in both small-sized IB4− (left panel) and small-sized IB4+ (right panel) TG neurons. Current injections of + 80 pA into the soma are shown in the top panels. *p < 0.05 and **p < 0.01 vs. sham, unpaired t-test