Fig. 5.
ES increased P/Q-type HVA channel currents in small-sized IB4− subpopulation of TG neurons. a and b, Representative current traces (a) and a summary of the results (b) demonstrating that ES significantly increased high voltage-activated (HVA) channel currents in the small-sized IB4− subpopulations of TG neurons but not in small-sized IB4+ TG neurons. *p < 0.05 vs. sham, unpaired t-test. To measure the HVA channel currents, neurons were held at − 60 mV and depolarized to 0 mV (10 mV increments) for 200 ms. c and d, The current densities of total and individual HVA channel subtypes at 0 mV in both the small-sized IB4− (c) and IB4+ (d) subpopulations of TG neurons in the sham and ES-treated groups. The whole-cell IBa through individual HVA channels in small-sized TG neurons was dissected by cumulatively applying 10 μM nifedipine, 2 μM ω-conotoxin-GVIA and 0.5 μM ω-agatoxin-IVA. The remaining Cd2+-sensitive currents were considered R-type currents. *p < 0.05 vs. sham, unpaired t-test