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. 2019 Sep 9;19:390. doi: 10.1186/s12870-019-1999-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Bimolecular fluorescence complementation (BiFC) assay and dual luciferase assay. a BiFC assay was performed to confirm the interaction between MabHLH1 and MaMybA or MaAN2 in Nicotiana benthamiana leaves. Bars: 25 μm. b Dual luciferase assay of MabHLH1 with or without MaMybA or MaAN2: effects on the transcriptional regulation of MaCHS, MaDFR, and AtDFR promoters, respectively, in N. benthamiana leaves. The results show promoter activity expressed as the ratio of the MaCHS, MaDFR, and AtDFR promoters, LUC to 35S: REN. Error bars indicate the standard deviations (SD) of average results