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. 2019 Sep 9;19:390. doi: 10.1186/s12870-019-1999-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Anthocyanin composition analysis of the leaves and corollas of the control, OE-MaAN2, and OE-MaMybA tobaccos. High-performance liquid chromatography analysis of anthocyanin extract (a), and its corresponding hydrolysate (b) in the leaves and corollas of the control, OE-MaAN2, and OE-MaMybA tobaccos. According to the chromatographic peak in the sequence, the standards are delphinidin 3-rutinoside, cyanidin 3-rutinoside, and pelargonidin 3-rutinoside (from left to right) in diagram a; and delphinidin, cyanidin, petunidin, pelargonidin, and malvidin (from left to right) in diagram b. The mass spectrum and structure patterns of delphinidin 3-rutinoside (c)