Table 1.

Composition and physico-chemical properties of zein nanoparticles.

Sample	Code	Zein (mg/ml)	Stabilizer (% w/v)a	Mean sizes (nm)	Polydispersity Index	Zeta potential (mV)
Surfactant free	A	0.2	–	450 ± 12	0.22 ± 0.15	-15 ± 1
	B	0.4	–	317 ± 12	0.27 ± 0.01	-3 ± 1
	C	0.6	–	146 ± 1	0.24 ± 0.01	6 ± 2
	D	0.8	–	135 ± 1	0.25 ± 0.01	9 ± 1
	E	1	–	126 ± 2	0.21 ± 0.01	14 ± 1
	F	2	–	101 ± 1	0.18 ± 0.01	21 ± 1
	G	3	–	151 ± 5	0.58 ± 0.04	28 ± 1
T80a	H	2	1.25	289 ± 22*	0.55 ± 0.07	10 ± 1**
	I	2	2.5	131 ± 1**	0.18 ± 0.01	12 ± 1**
	L	2	5	125 ± 3**	0.27 ± 0.01	11 ± 2**
PLX188b	M	2	1.25	102 ± 3	0.24 ± 0.03	22 ± 1
	N	2	2.5	100 ± 2	0.19 ± 0.01	34 ± 5*
	O	2	5	90 ± 1	0.15 ± 0.01	21 ± 2
SDc	P	2	1.25	105 ± 1	0.15 ± 0.01	-32 ± 2**
	Q	2	2.5	320 ± 106*	0.46 ± 0.05	-40 ± 1**
	R	2	5	>1000**	0.76 ± 0.17	-35 ± 5**

*p < 0.05, **p < 0.001 (with respect to the formulation F).

^aTween 80®.

^bPoloxamer188®.

^cSodium deoxycholate.