Fig. 4.

MBD1 induces EMT in GBC cancer cells and plays a role in the chemosensitivity of GBC cells. a MBD1 silencing resulted in increased expression of the epithelial marker E-cadherin and inhibition of mesenchymal markers, including Twist1, N-cadherin and Vimentin. b–d GBC cell chemosensitivity to Gem was evaluated in MBD1 knockdown cell clones. b The Gem sensitivity of GBC-SD cells with downregulation of MBD1 expression was significantly enhanced relative to that of control cells. c MBD1 knockdown elevated the Gem sensitivity of SGC-996 cells relative to that of control cells. d Inhibition of MBD1 expression dramatically decreased the IC50 of Gem in GBC-SD and SGC-996 cells. *P < 0.05, **P < 0.01