Fig. 3.

Effect of acetaldehyde-generating system (AGS) and hepatitis B virus (HBV) on proteasome subunit protein expression. Cells were treated or not with AGS for 72 h and with interferon-γ (IFNγ) for the last 24 h. A: proteasome activator PA28α and immunoproteasome subunits low-molecular-mass poylpeptide (LMP)2 and LMP7 protein expressions were detected by immunoblotting in cell lysates. Equal (20 µg) amounts of protein were loaded in each lane; β-actin was used as internal control. B, C, and D: quantification of immunoblotting bands from 3 independent experiments are presented as means ± SE. Bars marked with the same lowercase letter are not significantly different from each other; bars with different lowercase letters are significantly different (P ≤ 0 0.05).