Fig. 8.

Effect of acetaldehyde-generating system (AGS) on interferon-γ (IFNγ)-induced expression of transporter associated with antigen processing 1 (TAP1) and tapasin. HepG2.2.15 cells cells were treated or not with AGS for 72 h and with IFNγ for the last 24 h. A and C: TAP1 and tapsin protein expressions were detected by immunoblotting in cell lysates. Equal (20 µg) amounts of protein were loaded in each lane; β-actin was used as internal control. B and D: quantification of immunoblotting bands from 3 independent experiments presented as means ± SE. Bars marked with the same lowercase letter are not significantly different from each other; bars with different lowercase are significantly different (P ≤ 0 0.05).