Fig. 3.
Autophagy-related 5 (Atg5)-deficient club cells have a diminished apical protrusion and numbers of secretory granules. A–D: representative transmission electron micrographs of club cells at 2 different magnifications [scale bars = 2 µm (A and C) and 500 nm (B and D)] in Atg5f/f and secretoglobulin family 1A member 1 (Scgb1a1Cre+);Atg5f/f mice. White dashed lines in A and B demarcate apical protrusions of club cells. In C and D, large black arrows mark high-density granules (G) and small black arrows mark mitochondria (M). E: height of apical club cell cytoplasmic protrusion (measured from apex of cell to dashed white line). F: number of club cell granules in the apical protrusion. Values are means ± SE; n = 3 mice/group performed in 2 separate experiments. ns, Not significant.