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. 2018 Oct 8;11(6):509–520. doi: 10.1093/jmcb/mjy055

Figure 6.

Figure 6

Depletion of IRS2 attenuates NO production and eNOS knockdown reduces AKT activity. (A) NO production was measured by the conversion of L-arginine to NO in HUVECs transfected with the indicated siRNA for 48 h. (B–E) The protein levels of p-Akt and t-Akt detected by western blot analysis in HUVECs transfected with the indicated siRNA for 24 h, starved overnight, then treated with or without 20% FBS for 5 or 15 min. Data are mean ± SEM normalized to control. ANOVA followed by the Bonferroni post hoc test was used for analysis. n = 5, *P < 0.05 compared with control; #P < 0.05 compared with control; ns, no significance.