Table 1.

Primer Sequences for Fusion Confirmation and CRLF2 and EPOR Expression

Gene or gene fusion	PCR reaction	Forward primer	Reverse primer
ZNF274-JAK2	PCR1	5′-GGTTTTACCCCGGAAGAGTG-3′	5′-GCAGGAAGCTGATGCCTATC-3′
	PCR2	5′-TGTAAAACGACGGCCAGTAGGGAAGTGATGCTGGAGAA-3′	5′-CAGGAAACAGCTATGACCTGCAGATTTCCCACAAAGTG-3′
PAX5-ZCCHC7	PCR1	5′-ACACCAACAAGCGCAAGAGA-3′	5′-GGCTGGACAGGAATACAGGA-3′
	PCR2	5′-TGTAAAACGACGGCCAGTACTTCCTCCGGAAGCAGATG-3′	5′-CAGGAAACAGCTATGACCCAGATAATGTTTTTGTTGGCTGAA-3′
ATF7IP-PDGFRB	PCR1	5′-CCTTGCCAAATCCCACTAAA-3′	5′-TTTCATCGTGGCCTGAGAAT-3′
	PCR2	5′-TGTAAAACGACGGCCAGTGCCACAAGGACTTCTTTACCC-3′	5′-CAGGAAACAGCTATGACCCCTTCCATCGGATCTCGTAA-3′
CENPC-ABL1	PCR1	5′-ACAGAGCAAGGCCAGAATGT-3′	5′-ACACCATTCCCCATTGTGAT-3′
	PCR2	5′-TGTAAAACGACGGCCAGTTGTATTCAGTCACCAAGCAAAGA-3′	5′-CAGGAAACAGCTATGACCACGAAAAGGTTGGGGTCATT-3′
NUP153-ABL1	PCR1	5′-CATCTCTGACTCCTTCTGGTGA-3′	5′-CCACCGTCAGGCTGTATTTC-3′
	PCR2	5′-TGTAAAACGACGGCCAGTTGACACCCGGACAAAATAGAG-3′	5′-CAGGAAACAGCTATGACCGGGGGACACACCATAGACAG-3′
SPTAN1-ABL1	PCR1	5′-ACCGCTTCAAGGAACTCTCA-3′	5′-TGGTGTCCTCCTTCAAGGTC-3′
	PCR2	5′-GTAAAACGACGGCCAGTCAACCCTTAGGCGTCAGAAG-3′	5′-CAGGAAACAGCTATGACGGATAATGGAGCGTGGTGAT-3′
CRLF2	RT-PCR	5′-ATGCCAGCAAATACTCCAGGAC-3′	5′-AAGGTAGTTGGTGCACTGGTCAT-3′
EPOR	RT-PCR	5′-ATCCTCGTGGTCATCCTGGTG-3′	5′-GGATGCCAGGCCAGATCTTC-3′
ACTB	RT-PCR	5′-CCTGAACCCCAAGGCCAAC-3′	5′-ACAGCCTGGATAGCAACGTACA-3′