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. 2019 Aug 28;2019:9219847. doi: 10.1155/2019/9219847

Figure 3.

Figure 3

ERK/MEK signaling pathway is inactivated and then activated following hyperoxia. (a) Expression bands of phospho-ERK (p-ERK), ERK, phospho-ERK (p-MEK), and MEK in the kidneys of newborn rats exposed to normoxia or hyperoxia as detected by western blotting. Relative expression adjusted to the reference gene β-actin and then standardized to the value of the normoxia group on P1D is shown by box and whisker plots. The whiskers represent the minimal or the maximal gray value, and the boxes span the interquartile range of measurements for 10 rats with the mean value of 3 replicates (n = 10). ∗P < 0.05, one-way ANOVA, Bonferroni post hoc test. (b) Expression bands of hypoxia-inducible factor 1α (HIF-1α) and catalase in the kidneys of newborn rats exposed to normoxia or hyperoxia and detected by western blotting. Relative expression adjusted to the reference gene GAPDH and then standardized to the value of the normoxia group on P1D is shown by box and whisker plots. The whiskers represent the minimal or the maximal gray value, and the boxes span the interquartile range of measurements for 10 rats with the mean value of 3 replicates (n = 10). ∗P < 0.05, one-way ANOVA, Bonferroni post hoc test.