Table 1.
Fluorescence quantitative real-time PCR results of control and experimental groups.
Sample description | β-actin | Fth1 | ΔCT | ΔΔCT | 2−ΔΔCT |
---|---|---|---|---|---|
Control | 18.52 | 28.01 | 9.49 | 0 | 1 |
Fth1 | 18.12 | 23.49 | 5.37 | −4.11 | 17.34 |
Note: the quantitative assay of each sample was conducted thrice; using internal parameters, the relative quantities of mRNA were calculated using the 2−ΔΔCT algorithm. 2−ΔΔCT represents the consequence. By the standard of cells responding to EGFP-Fth1 viral infection, 2−ΔΔCT was calculated.