Table 2.
TCRβ sequences of porcine TRAV1-TRAJ33+ cells.
| TRAV-TRAJ | TRBV | CDR3β | TRBJ | Number |
|---|---|---|---|---|
| TRAV1-TRAJ33 | TRBV29S | SAGYIRGAGGNTQHF | Jβ2.4 | 8 |
| TRAV1-TRAJ33 | TRBV29S | SAGYIRVAGGNTQHF | Jβ2.4 | 2 |
| TRAV1-TRAJ33 | TRBV29S | SAGDWVPLYSETQYF | Jβ2.5 | 1 |
| TRAV1-TRAJ33 | TRBV20S | ATSYSQTQYF | Jβ2.5 | 1 |
| TRAV1-TRAJ33 | TRBV20S | GAKRALTEGNTQHF | Jβ2.4 | 2 |
| TRAV1-TRAJ33 | TRBV20S | GASVWGGDTEVFF | Jβ1.1 | 2 |
| TRAV1-TRAJ33 | TRBV20S | GASNSRGAGTDPLYF | Jβ2.3 | 1 |
| TRAV1-TRAJ33 | TRBV20S | ALGQGRADPLYF | Jβ2.3 | 1 |
| TRAV1-TRAJ33 | TRBV20S | ASGRRGSETQYF | Jβ2.5 | 1 |
| TRAV1-TRAJ33 | TRBV20S | ARQRGTGLSGYAEQHF | Jβ2.1 | 1 |
| TRAV1-TRAJ33 | TRBVX | FEDNYAEQHF | Jβ2.1 | 1 |
| TRAV1-TRAJ33 | TRBVX | SEHGGAAQLYF | Jβ2.2 | 2 |
| TRAV1-TRAJ33 | TRBVX | SDWIGKSLGSTQHF | Jβ2.4 | 1 |
| TRAV1-TRAJ33 | TRBVX | SEDSWGSPTAEQHF | Jβ2.1 | 1 |
| TRAV1-TRAJ33 | TRBV7S | RQGLTDPLYF | Jβ2.3 | 2 |
| TRAV1-TRAJ33 | TRBV7S | LRWRDLNYNSPLHF | Jβ1.6 | 1 |
| TRAV1-TRAJ33 | TRBV4S | RGRGGYDYNF | Jβ1.2 | 1 |
| TRAV1-TRAJ33 | TRBV4S | PAGGIAGSPLHF | Jβ1.6 | 1 |
| TRAV1-TRAJ33 | TRBV5S | LDAMGQTQYF | Jβ2.5 | 1 |
| TRAV1-TRAJ33 | TRBV5S | PAFGQSRYPLYF | Jβ2.3 | 1 |
| TRAV1-TRAJ33 | TRBV11S | SRRDTNEKLIF | Jβ1.4 | 1 |
| TRAV1-TRAJ33 | TRBV11S | QHPRGGGSPSYEQIF | Jβ2.7 | 1 |
| TRAV1-TRAJ33 | TRBV10S | MGGQTQYF | Jβ2.5 | 1 |
| TRAV1-TRAJ33 | TRBV12S | ETVNRNTGQLYF | Jβ2.2 | 1 |
| TRAV1-TRAJ33 | TRBV15S | RDRAMGYSQTQYF | Jβ2.5 | 1 |
| TRAV1-TRAJ33 | TRBV19S | PWLRGASQNTQHF | Jβ2.4 | 1 |
| TRAV1-TRAJ33 | TRBV25S | GDRGENTQHF | Jβ2.4 | 1 |
| TRAV1-TRAJ33 | TRBV27S | VSDRGITDPLYF | Jβ2.3 | 1 |
| TRAV1-TRAJ33 | TRBV30S | WVGADSYEQIF | Jβ2.7 | 1 |
αβ T cells from porcine lung were sorted as single cells into 96-well plate, and then nested PCR was performed to identify TRAV1-TRAJ33+ cells, followed by amplifying TCRβ sequences from these TRAV1-TRAJ33+ cells. The named principle of Vβ and Jβ genes was according to the nomenclature used by Butler et al. (48). CDR3β was defined in accordance with Kabat et al. (52).