Figure 2.

Diarrhea frequencies over time following vitamin D treatment of C. jejuni infected mice. Secondary abiotic IL-10^−/− mice were treated with synthetic 25-OH-cholecalciferol (vitamin D, VitD, white bars) or placebo (PLC, black bars) via the drinking water starting 4 days before peroral C. jejuni 81-176 strain infection on days 0 and 1. Occurrence of diarrhea was assessed in each mouse from day 0 until day 6 post-infection as indicated applying a standardized clinical scoring system (see Materials and Methods). Bars indicate the cumulative frequencies of diarrhea (in %). Numbers of diarrheal mice out of the total number of analyzed animals are given in parentheses. Data were pooled from four independent experiments.