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. 2019 Sep 3;10:1125. doi: 10.3389/fphys.2019.01125

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Copyright © 2019 Wang, Cao, Wen, Xiao, Chen, Guan, Liu, Zuo, Qian, Deng and Xin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Myocardial cells senescence and ROS production were increased by D-gal. (A) The expression of CD38 by real-time PCR analysis in H9c2 cells treated with different concentrations of D-gal. (B) SA-β-gal staining in H9c2 cells treated with D-gal (10 g/L). (C) The images of senescence marker p16, p21 and CD38 protein by Western blot analysis in H9c2 cells treated with D-gal (10 g/L). (D) Quantitative analysis of p16, p2,1 and CD38 protein level from western blot bands. (E) The mean fluorescence intensities of ROS production were quantitatively analyzed in H9c2 cells treated with D-gal. Data are shown as mean ± SEM, ^∗p < 0.05, ^∗∗p < 0.01 and ^∗∗∗p < 0.001, n = 3 per group.