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. 2019 Sep 3;10:2029. doi: 10.3389/fmicb.2019.02029

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Copyright © 2019 Ma, He, Yu, Cai, Zeng, Cai, Lu, Chen, Chen and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A–C) Polymyxin B -resistant subpopulation CFU and total CFU of B1, D1, and D4 during 24 h treatment with 16 mg/L polymyxin B in liquid culture and subculture without polymyxin B for further 24 and 72 h (n = 3). (D–F) qRT-PCR analysis of the phoP, phoQ, mgrB, pmrA, pmrB, pmrC, and acrB gene expression in resistant and susceptible subpopulations of B1, D1, and D4. Relative abundance was calculated by normalizing the expression of each gene to the average expression of the housekeeping gene, rpoB (n = 4). Statistically significant: ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001. n.s., not significantly different (unpaired t-test).