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. 2019 Sep 5;41(1):821–831. doi: 10.1080/0886022X.2019.1655450

Figure 7.

Figure 7.

The ACSL1 expression was regulated by Nrf2. Human proximal tubular epithelial cells (HK-2) were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum, 0.5% penicillin and streptomycin in 5% CO2 incubator at 37 °C. For transfection experiment, transfection of siRNA (100 nM) or plasmid (2500 ng), and then the HK-2 cells were treated with or without palmitic acid (PA) (0.04 mmol/l) for 24 h. (A) DHE staining of HK2 cells 200×. (B) Western Blot for ACSL1. (C) Densitometry analysis is presented as relative ratios of ASCL1/actin. The data are means ± SE (n = 4); *p < 0.05 compared with control; #p < 0.05 compared with PA group. (D) Quantitative analysis of intracellular FFA and TG contents in HK2 cells. The data are means ± SE (n = 4). *p < 0.05 versus the control group. #p < 0.05 compared with PA group.