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. 2019 Sep 5;41(1):821–831. doi: 10.1080/0886022X.2019.1655450

Figure 8.

Figure 8.

ACSL1 was the direct role in renal lipid deposition in ORN. Human proximal tubular epithelial cells (HK-2) were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum, 0.5% penicillin and streptomycin in 5% CO2 incubator at 37° C. For transfection experiment, transfection of siRNA (100 nM) or plasmid (2500 ng), and then the HK-2 cells were treated with or without palmitic acid (PA) (0.04 mmol/l) for 24 h. (A) Western blot for ACSL1. (B) Quantitative analysis of intracellular FFA and TG contents in HK2 cells. Data are expressed as mean ± SE from four independent experiments. *p < 0.05 versus the control group; #p < 0.05 versus the PA group. $p < 0.05 versus the PA + Nrf2-plasmid group. (C) Quantitative analysis of intracellular FFA and TG contents in HK2 cells. Data are expressed as mean ± SE from four independent experiments. *p < 0.05 versus the control group; #p < 0.05 versus the PA group. $p < 0.05 versus the PA + Nrf2-siRNA group.