Table 1.
Inhibitory effects of compounds 1–6 on α-glucosidase.
| Compounds | α-Glucosidase |
|||
|---|---|---|---|---|
| IC50 (μM)a | Kinetic mode (Ki, μM)b | KI (μM) | KIS (μM) | |
| 1 | 7.6 ± 0.2 | Competitive (2.9 ± 0.4) | NTc | NT |
| 2 | 8.6 ± 0.5 | Competitive (5.1 ± 0.3) | NT | NT |
| 3 | 25.4 ± 0.7 | Competitive (12.6 ± 0.7) | NT | NT |
| 4 | 9.6 ± 0.6 | Competitive (5.8 ± 0.4) | NT | NT |
| 5 | 8.8 ± 0.3 | Mixed type I (5.4 ± 0.7) | 5.4 | 20.4 |
| 6 | 16.2 ± 0.8 | Mixed type I (15.8 ± 0.9) | 15.4 | 51.7 |
| DNJd | 42.5 ± 0.9 | NT | NT | NT |
a
Sample concentration which led to 50% enzyme activity loss.
b
Ki is the inhibition constant.
c
NT is not tested.
d
Deoxynojirimycin (DNJ) is used as a positive control.