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. 2019 Aug 12;9(20):5769–5783. doi: 10.7150/thno.36505

Figure 1.

Figure 1

The Level of HSP90 paralogs in the Liver of Obese Mice and NAFLD Patients. (A) The immunohistochemistry staining of HSP90β or HSP90α in the liver of healthy donors (n=5) and NAFLD patients (n=20). (B) The correlation between immunostaining signal of HSP90s and the clinical parameters including serum level of glucose, insulin, TG and TC (n=20). (C, D, G, H) Liver sections of HFD-induced obese mice (C, D) or ob/ob mice (G, H) were stained with HSP90 paralog specific antibodies. (D and H) The mRNA level of HSP90AB1 or HSP90AA1 in the liver was analyzed in HFD- induced obese mice (E) and ob/ob mice (I), respectively. n = 9 for chow group or HFD group; n = 14 for WT mice group, n = 8 for ob/ob mice group. (F and J) The protein level of HSP90α or HSP90β in the liver was analyzed by western blot in HFD- induced obese mice (E) and ob/ob mice (I), respectively. Error bars are represented as mean ± SEM. The Pearson correlation coefficient is used to test the correlation between HSP90 paralogs expression and clinical parameters (B). Student t-test was used to analyze significance between two sets of data (D and F). ***p < 0.001 vs chow diet mice or WT mice.