Figure 1.

Generation 3.0 (G3) glyco-dendrimers efficiently target both DC-SIGN and Langerin. DC-SIGN and Langerin targeting was evaluated for different glyco-particles using CLR-Fc or a cell line transduced with DC-SIGN or Langerin A. Schematic representation of (glyco)-dendrimer synthesis. B. Binding of G0 (upper panels) or G3 (lower panels) (glyco)-dendrimers to human DC-SIGN-Fc (left panel) or Langerin-Fc (right panel) in the presence or absence of calcium depletion (EGTA) as measured by binding ELISA. C. Binding to membrane DC-SIGN and Langerin of different (glyco)-particles within 1 hour at 37 degrees; G0 (grey) or G3 (pink) (glyco)-dendrimers and (glyco)-liposomes (blue). D. DC-SIGN and Langerin specific binding by G3 glyco-dendrimers was evaluated using specific blocking antibodies for DC-SIGN and Langerin, or matched isotype control, prior to incubation. Data are representative of at least two independent experiments measured in triplicate ±SD (Statistical analysis: B two-way ANOVA Sidak's post hoc, C-D two-way ANOVA Tukey's post-hoc)