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. 2019 Aug 14;9(20):5976–6001. doi: 10.7150/thno.33959

Figure 10.

Figure 10

Expression of Gal-3 on TLR-2-primed DCs is crucially important for their capacity to enhance nephroprotective and immunosuppressive properties of Tregs. For the depletion of Tregs, anti-CD25 monoclonal antibody (250 μg/mouse) was intraperitoneally given to mice 3 days before CDDP administration (16 mg/kg body weight). For transfer experiments non-primed Tregs, Tregs primed with WTDCPam3CSK4, WTDCPam3CSK4+Davanat or Gal-3-/- DCPam3CSK4 (1x106 Tregs/ mouse) were intravenously injected in CDDP-treated animals 18 h before induction of AKI. Significantly lower serum levels of urea and creatinine (A) and reduced histological score accompanied with attenuated renal injury, observed in the cortex of CDDP-injured kidneys (B) were observed in Treg-depleted CDDP-treated mice that received WTDCPam3CSK4-primed Tregs compared to the Treg-depleted CDDP-injured animals that received non-primed Tregs. There was significantly lower number of T-bet-expressing, IFN-γ-, IL-17- and TNF-α-producing neutrophils (C-F) and remarkably higher number of IL-10-producing neutrophils (G) in the kidneys of Treg-depleted CDDP-treated mice that received WTDCPam3CSK4-primed Tregs. Significantly higher serum concentrations of urea and creatinine (A), more severe injury of proximal tubular epithelial cells and increased histological score (B), significantly higher number of inflammatory neutrophils (C-F) and significantly reduced number of immunosuppressive, IL-10-producing neutrophils (G) in the kidneys of Treg-depleted CDDP-treated mice that received Gal-3-/-DCPam3CSK4 or WTDCPam3CSK4+Davanat -primed Tregs compared to Treg-depleted CDDP-treated mice that received WTDCPam3CSK4-primed Tregs. Individual data points with Mean, obtained in one experiment with 6 mice per group; *p<0.05, **p<0.01,***p<0.001.