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. 2019 Aug 14;9(20):5976–6001. doi: 10.7150/thno.33959

Figure 4.

Figure 4

Gal-3 deficiency significantly increased total number of inflammatory Th1 and Th17 cells and reduced presence of regulatory T cells in CDDP-injured kidneys. Bar graphs (left panels) and representative density plots obtained by flow cytometry analysis and intracellular staining of renal-infiltrated CD4+T cells derived from saline or CDDP-treated WT and Gal-3-/- mice, 72 h after CDDP administration (16 mg/kg body weight). Gal-3 deficiency resulted with increased presence of CD4+T cells in the CDDP-injured kidneys (A, left panel). Representative density plots showing CD4+ T lymphocytes, gated as CD4+ cells in population of renal infiltrated cells (A, right panel). Significantly higher total number of T-bet-expressing (B) and IFN-γ-producing (C) CD4+Th1 cells and RORγT-expressing (D) and IL-17-producing (E) CD4+Th17 cells, but reduced presence of immunosuppressive IL-10-producing CD4+T cells (F), CD4+FoxP3+ Tregs (G) and STAT-3-expressing Treg (H) were noticed in the kidneys of CDDP-treated Gal-3-/- mice. Representative density plots showing percentages of T-bet-expressing, IFN-γ-producing, RORγT-expressing, IL-17-producing, IL-10-producing, FoxP3-expressing cells, gated in the population of CD4+Tcells (B-G, right panels) and percentage of STAT-3-expressing cells in gated population of CD4+FoxP3+ Tregs (H, right panel), isolated from the kidneys of CDDP-treated WT and Gal-3-/- mice, 72h after CDDP administration. Real-time PCR gene analysis showing significantly higher mRNA expression of T-bet, IFN-γ, RORγT, IL-17 and lower mRNA expression of FoxP3 in CDDP-injured kidneys of Gal-3-/- mice compared to similarly treated WT animals (I). Data from two individual experiments with 8 mice per group are shown as Mean ± SEM; *p<0.05, **p<0.01,***p<0.001.