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. 2019 Jul 28;9(19):5444–5463. doi: 10.7150/thno.29367

Figure 4.

Figure 4

Protein expression analysis of Clover-PPARγ1 and N-CoR2-mRuby2 constructs. Graphical scheme of the structure of the full-length human N‑CoR2 protein (A) and the used N-CoR2 constructs, N-CoR2 WT-mRuby2, N-CoR2 (ΔID1 exon)-mRuby2 (amplified out of HEK293T cells cDNA), N-CoR2 (ΔID1 CoRNR box)-mRuby2 and N-CoR2 (ΔID2 CoRNR box)-mRuby2 (B). All N-CoR2 constructs were C-terminally labeled with the red fluorophore mRuby2. Western blot analysis of total lysate of HEK293T cells stably expressing Clover-PPARγ1, N-CoR2 WT-mRuby2, N-CoR2 (ΔID1 exon)-mRuby2, N-CoR2 (ΔID1 CoRNR box)-mRuby2 and N-CoR2 (ΔID2 CoRNR box)-mRuby2 alone or in combination against human PPARγ1 (C), GFP (D), human N-CoR2 (E) and tRFP (F). Images are representative of experiments which were performed at least three times.