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Transfer of the flow cytometry-based FRET system to J774A.1 macrophages. Flow cytometry-based FRET efficiency was determined in murine J774A.1 macrophages following transduction with (A) Clover + mRuby2 or Clover fused (63bp) mRuby2 and (B) Clover-PPARγ1 in combination with N-CoR2 WT-mRuby2 or N-CoR2 (ΔID exon)-mRuby2. Flow cytometry-based FRET efficiencies of Clover/mRuby2-double positive cells were calculated as described in Materials and Methods.
