Figure 6.

STAT3 KO downregulates expression of genes involved in the G2/M phase. (A). Cell cycle profiling revealed G2/M enrichment in STAT3 KO cells. Cells were fixed, stained with propidium iodide, and DNA content was analysed by flow cytometry. Statistical analysis is provided in Supplemental Table S8. (B) Key cell cycle mediators coding genes were mostly down regulated in SKOV3 STAT3 KO cells compared to WT cells. Colors represented log2 fold change differences between STAT3 KO cell lines and parental cell lines. The impact of STAT3 KO varied greatly in the magnitude of differential expression between Bru-seq and RNA-seq, two scale bars are used. (C). Key cell cycle mediators were suppressed in STAT3 KO cells. Protein expression levels were determined by Western blot.