Skip to main content
. 2019 Jul 28;9(19):5577–5594. doi: 10.7150/thno.34663

Figure 2.

Figure 2

CPX-induced downregulation of DJ-1 is involved in anti-CRC effects of CPX. A. Volcano plot analysis of DEGs in HCT116 and SW480 cells treated with or without 20 μM CPX for 24 hours. Red areas indicate significant overexpression and green areas indicate significant downregulation. B. qRT-PCR was conducted in HCT116 and SW480 cells treated with or without 20 μM CPX for 24 hours to determine DJ-1 mRNA expression. Statistical significance compared with respective control groups. C. Immunoblot analysis of DJ-1 expression in CRC cells treated with or without the indicated concentration of CPX for 24 hours. D and E. Colony formation assay of CRC cells stably overexpressing DJ-1 and Vector treated with indicated concentrations of CPX. F. Cell viability assay was performed by CCK8 kit. G-J. HCT116 cells stably overexpressing DJ-1 and Vector were subcutaneously injected into nude mice(n=6). Tumor volumes were monitored at indicated time points (G), and the weight of tumors were measured at time of sacrificed (H). (I) The image of isolated tumors derived from mice treated with vehicle or CPX (25 mg/kg/day). (J) Representative images of immunohistochemical staining of PCNA and quantification of relative intensity of PCNA staining in xenografts. Scale bar, 100 μm. *, P < 0.05, **, P < 0.01, ***, P < 0.001.