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Molecular mechanism induced by Au-Apt-TPE@Zn. (A) Real-time ROS regeneration. ROS generation was detected by DCFH-DA probe as described in method section. (B) Relative cDNA abundance. The relative cDNA abundance of akt, p38, Jnk and p53 were detected by real-time RT-PCR. (C) Western blotting for protein expression. MAPKs, PI3K/AKT and DNA damage signal molecular were examined by western blotting method. Data are means ± SD, * P<0.05, ** P< 0.01, vs. blank group.
