FIGURE 5.

MSC homing to (A) kidney and (B) muscle at 24 hours post-pFUS. Mice received IV-infusions of 10⁶ human MSC 4 h post-pFUS and then were euthanized the following day. Kidney and muscle histology were stained for human mitochondria (red) to detect and quantify MSC (nuclei shown in blue). MSC were quantified in 10 fields-of-view in each of 3 histological slices per mouse from 3 mice. pFUS significantly increased MSC homing to wild-type kidneys and muscles, but homing was significantly suppressed in wild-type mice that received intravenous GdCl₃ (300 μg/kg), Ruthenium Red (RR; 22 mg/kg), or intraperitoneal verapamil (5 mg/kg) prior to pFUS and untreated TRPC1-KO mice. Mice treated with GdCl₃ had significantly more cells than control mice (no pFUS), but still had significantly fewer MSC than pFUS-treated mice that received no drug (*, ⁺ = p<0.05 by ANOVA) (scale bars represent 100 μm for kidney images and 50 μm for muscle images). For presentation, control samples represent values from unsonicated tissues in mice that received no drugs or TRPC1-ko. These values were not significantly different from unsonicated tissues in mice that received drugs or TRPC1-ko (See Supplemental Data).