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COX2 upregulation by pFUS in TCMK1 and C2C12 cells is dependent on intracellular Ca2+. Cultured TCMK1 or C2C12 cells were treated with pFUS and then immunostained for COX2 (green) 24 hours post-treatment (nuclei shown in blue). pFUS upregulated COX2 in both cell types and upregulations were blocked when both cell types were loaded with BAPTA-AM prior to pFUS (scale bars represent 10 μm).
