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. 2019 Jul 28;9(19):5517–5531. doi: 10.7150/thno.33876

FIGURE 7.

FIGURE 7

TRPC1 and LTCC form complexes without ORAI1 in kidneys and muscle. Confocal imaging for TRPC1, LTCC, and ORAI in (A) kidney and (B) muscle. Identical intensity thresholds were used for all groups within each tissue type to determine pixel colocalization (quadrant 3 in each image). Colocalized pixels for each comparison are shown in red and background pixels shown in grey. Comparing TRPC1 and LTCC reveals colocalizations on what appears to be the apical surfaces of tubular epithelium and the plasma membrane of skeletal muscle fibers. Substantial colocalization of TRPC1 and ORAI1 is observed, but in different cellular regions than the TRPC1/LTCC pixels. Lastly, there is essentially no colocalization between LTCC and ORAI1 (scale bars represent 20 μm). C) Immunoprecipitation of the LTCC from both tissue types precipitates associated TRPC1 molecules, but not ORAI1 molecules.