. 2019 Jul 31;141(34):13468–13478. doi: 10.1021/jacs.9b04823

Table 3. Effect of Q215A, Y217F, and R235A Substitutions on the Activation Barriers for ScOMPDC-Catalyzed Decarboxylation of OMP at pH 7.1 and for Exchange of the C-6 Hydrogen of FUMP for Deuterium in D₂O at pD 8.1.

enzyme	ΔΔG_OMP^‡ kcal/mol^b	ΔΔG_FUMP^‡ kcal/mol^b	[ΔΔG_FUMP^‡ – ΔΔG_OMP] kcal/mol
Q215A	2.2	3.5	1.3
Y217F	2.4	4.1	1.7
Q215A/Y217F	4.8	6.8	2.0
R235A^a	5.6	7.2	1.6

Data from ref (12).

The effect of the amino acid substitution(s) on the activation barrier ΔG^‡ for k_cat/K_m for ScOMPDC-catalyzed decarboxylation of OMP, or on k_ex/K_d for ScOMPDC-catalyzed exchange of the C-6 H of FOMP for deuterium, calculated from data in Table 1.

Table 3. Effect of Q215A, Y217F, and R235A Substitutions on the Activation Barriers for ScOMPDC-Catalyzed Decarboxylation of OMP at pH 7.1 and for Exchange of the C-6 Hydrogen of FUMP for Deuterium in D2O at pD 8.1.

Table 3. Effect of Q215A, Y217F, and R235A Substitutions on the Activation Barriers for ScOMPDC-Catalyzed Decarboxylation of OMP at pH 7.1 and for Exchange of the C-6 Hydrogen of FUMP for Deuterium in D₂O at pD 8.1.