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. 2019 Apr 6;15(10):1774–1786. doi: 10.1080/15548627.2019.1596478

Figure 1.

Figure 1.

Golgi-derived RAB2+ vesicles fuse into autophagic membrane structures by vesicular trafficking. (a) Western-blot (WB) analysis of FLAG-RAB2 stable cell lines using anti-RAB2 antibody. (b) WB analysis of GFP-RAB2 and endogenous RAB2 level using anti-RAB2 antibody. (v) The subcellular localization of mCherry-RAB2 was similar to the stably expressed FLAG-RAB2. (d) Confocal microscopy analysis of GFP-RAB2 with TGOLN2 and LC3 as indicated under untreated, Torin1- or Torin1 plus nocodazole-treated conditions. Scale bars: 10 µm. (e) Quantification of colocalization presented in Figure 1 (d) and S1. Data were shown as mean ± SD, *p < 0.05, **p < 0.01; ‘ns’ indicates no statistical significance. (f) Schematic representation of the trafficking routes of RAB2. Red arrow heads indicated the routes uncovered in this work.