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. 2019 Aug 14;9(21):6099–6111. doi: 10.7150/thno.36790

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Fig 3 — CRISPRa-engineered ASC sheets promoted SC migration. (A) SC migration induced by the CRISPRa-engineered ASC sheets. (B) Number of SC migrating through the membrane. Rat ASC sheets cultured in 6-well plates were co-transduced with Bac-Cre and Bac-LECW at MOI 100/150 (CRISPRa group) or mock-transduced (Mock group) and then co-cultured with rat SC in the transwell assay. Two days after co-culture, the porous inserts were stained with crystal violet. Four fields in each insert were randomly chosen and the numbers of migrating SC were counted using ImageJ software. The data represent means±SD of 3 independent culture experiments.