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. 2019 Aug 14;9(21):6224–6238. doi: 10.7150/thno.36281

Figure 5.

Figure 5

RNAseq analysis of tumors from BRCA-deficient mice treated with NanoTLZ or i.v. Talazoparib. When Brca1Co/Co;MMTV-Cre;p53+/- mice developed tumors 4 mm in diameter, they were treated with either saline, NanoTLZ (0.33 mg/kg), or Talazoparib (i.v. TLZ, 0.33 mg/kg) by i.v. for 5 doses (3 times a week). Total RNA was isolated from the tumors and processed for RNAseq analysis. A. Cluster analysis of differentially expressed genes in each group. N=3 mice/group. B. Venn diagram of differentially expressed genes in NanoTLZ and i.v. Talazoparib group. C. Validation of gene expression by real-time PCR. Data presented as mean ± SEM. N=5 mice/group. *, p<0.05 vs. Saline. D. Protein expression of PCNA, CyclinD1, CyclinE1, cleaved-caspase (c-caspase) 3 in tumors treated with saline or NanoTLZ. Vinculin was used as the loading control. E. Immunohistochemistry of PCNA and γH2AX expression in tumor sections, 400x magnification.