Figure 4.
Enhanced mitochondrial fusion partially rescued mitostasis albeit with no enhanced proteome stability. (a) Stereoscope viewing of 3rd instar control (+/Gal4-Tub) or transgenic larvae expressing the indicated transgenes. (b) Stereoscope viewing of control adult flies’ eyes (+/Gal4-GMR) or after targeted eye expression of the indicated transgenes. (c, d) CLSM viewing of Mito-GFP reporter along with immunofluorescence staining of tissues with a ref(2)P antibody and DAPI, in muscle (c) or nervous tissues (d; only Mito-GFP is shown) after KD of Prosβ5 or both the Prosβ5 and Marf genes. (e) CLSM visualization of Mito-GFP reporter in muscles of the shown transgenic larvae stained also for ref(2)P and counterstained with DAPI. (f) Relative mitochondrial ST3:ST4 ratio in tissues of the shown transgenic larvae. (g, i) Immunoblotting analyses of tissues protein samples from indicated transgenic larvae; samples were probed with antibodies against ref(2)P, Hsp70, ubiquitinated (Ub) (g) and carbonylated (DNP) (i) proteins. (h) Relative expression (vs. control) of Hsp70 and ref(2)P genes in 3rd instar control (+/Gal4-Tub) or transgenic larvae expressing the shown transgenes. If not otherwise indicated, data refer to 3rd instar stage larvae not exposed to RU486 (driver, Gal4-Tub). Arrows in (c), (e) indicate ref(2)P colocalization with aggregated mitochondria. Gapdh (g, i) probing was used as input reference. Bars, ± SD; n ≥ 2; *P < 0.05; **P < 0.01.