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. 2019 Apr 19;15(10):1757–1773. doi: 10.1080/15548627.2019.1596477

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 7. — Atg8a OE in adult flies suppresses the toxic effects of proteasome KD and increases adult flies’ longevity. (a) Immunoblotting analyses of protein samples from shown transgenic flies somatic tissues; samples were probed with antibodies against GFP (denotes expression of the GFP-Atg8a transgene), ubiquitinated (Ub) and carbonylated (DNP) proteins. (b) Immunoblotting analyses of protein samples from shown transgenic flies somatic tissues; samples were probed with antibodies against ubiquitinated (Ub) and carbonylated (DNP) proteins. (c) Longevity curves of indicated transgenic fly lines. Statistics of the shown longevity curves are reported in Table S1. In (a) young flies were exposed to RU486 for 3 days and were then treated (or not) with RU486/1 μΜ PS-341 for 4 days, while in (b) young flies were exposed to RU486 for 7 days. Gapdh (a, b) probing was used as input reference.