Figure 4.

Reactivity of recombinant proteins with serum samples of confirmed leptospirosis and of other unrelated diseases. Recombinant proteins (a) rLIC11711 and (b) rLIC12587 were immobilized on 96 well plates (250 ng/well). Reactivity was assessed by total IgG antibodies in human paired sera collected from patients at the onset (MAT-) and convalescent phase (MAT+), depicted by the same color, detected by incubation with HRP-conjugated anti-human IgG antibodies (1:5000). The cutoff values represented by the horizontal dashed line were defined as the mean plus three SD of the absorbance values from normal human serum (NHS).