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. 2019 Aug 9;34(9):1621–1631. doi: 10.1093/humrep/dez131

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© The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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MSC characteristics of HLA-A,B,C ⁺ /CD34 ⁻ /PDGFRα ⁺ cells. PDGFRα⁺ cells are positive for MSC minimal criteria markers CD90, CD73 and CD105 (blue) compared to IgG controls (gray) (A). Staining for trilineage differentiation of HLA-A,B,C⁺/CD34⁻/PDGFRα⁺ cells into adipocytes (Oil red O) (B), osteocytes (alizarin red) (C) and chondrocytes (alcian blue) (D). Scale bar is 50 μm. Relative gene expression of CFD for adipocytes, SPARC for osteocytes and COL10A1 for chondrocytes (E). Error bars indicate technical variation (n = 3). MSC: mesenchymal stromal cell; CFD: complement factor D; SPARC: secreted protein acidic and cysteine rich; COL10A1: collagen, type X, alpha 1.