Figure 5.

Androstenedione and progesterone production in vitro. Androstenedione levels were only detectable in two differentiation samples, stimulated with forskolin and dbcAMP (A). These two samples were both from cells from the same patient. Progesterone was found in several medium samples (B), with a significantly increased production of progesterone when cells were stimulated with dbcAMP compared to basal and hCG (asterisk (*) P < 0.05 using Tukey post-hoc test). Furthermore, a significant increased production was found when cells were cultured in differentiation medium compared to propagation medium (a and b differ significantly from each other (P < 0.05) by a two-way ANOVA).