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. 2019 Jun 28;47(15):8036–8049. doi: 10.1093/nar/gkz553

Figure 2.

Figure 2.

(A) Serotype specific SRCs expressed in the WT serotype (replicates were merged) samples were overlapped with annotated regions. Each annotated element is counted only once (distinct counting) and the number of elements of the different types is shown on the y-axis for all four serotypes. (B) sRNA reads (log10) in SRCs overlapping different genomic annotations (rows) and restricted to small RNA length (x-axis) for 51A serotype. (C) Length distribution of sense and antisense sRNAs mapping to protein-coding genes in 51A serotype. (D) Set intersection plots for SRCs overlapping with genes across serotypes. Genes consistently overlapping in the four WT serotypes, are called Genes associated with SRCs (GSRCs). (E) Boxplot of mRNA expression (y-axis, log2 TPM) in the four WT serotypes of GSRCs and other expressed genes. (F) Boxplot of total sRNA reads (y-axis; log2) in the EEJ and introns of GSRCs. (G) A histogram of the number of GSRCs (y-axis) plotted against the overlap percentage (in basepairs) of a GSRC by one or more SRCs (x-axis) is shown here. Based on the overlap percentage, GSRCs were split into three classes as shown (vertical lines) for further analysis. *P < 0.05 with a two-tailed Wilcoxon test.