Figure 6.

Visualization of DNA damage with corresponding fit to the human genome. Examples of DNA molecules extracted from PBMCs and exposed to the chemotherapeutic agent etoposide. The DNA damage sites are visualized in green (ATTO-647), and the barcode based on the underlying DNA-sequence in blue (YOYO-1/Netropsin). (A) Comparison between experimental (blue) and theoretical (black) barcodes, demonstrating excellent overlap and a P-value < 10⁻⁶ (top), as well as the normalized intensity in the 647 channel along the center line of the DNA-molecule displaying the DNA-damage sites (green) (bottom). (B) Microscopy images of YOYO-1 channel (blue, top), ATTO-647 (green, center) and overlay of both channels (composite, bottom). The optimal focus will shift slightly for the different emission wavelengths of YOYO-1 and ATTO-647. The left molecule shows an example where the focus was adjusted to the fit the green channel (ATTO-647) and the right molecule an example where the focus was adjusted to fit the blue channel (YOYO-1).