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. 2019 Jun 26;6(9):ofz306. doi: 10.1093/ofid/ofz306

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© The Author(s) 2019. Published by Oxford University Press on behalf of Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Phylogenetic analysis of 19 hepatitis E virus (HEV) strains isolated from domestic pigs. (A) Phylogenetic tree was constructed based on the alignment of 306 base pairs of the HEV RNA-dependent RNA polymerase (RdRp) region (ORF1) of 19 nucleotide sequences isolated from domestic pigs, 1 HEV strain isolated from a liver cancer patient coinfected with hepatitis B virus in Vietnam. Forty full-length HEV genomes isolated from animals and humans (HEV-1 to HEV-8) retrieved from the NCBI database along with GenBank accession numbers were included in the analysis. A neighbor-joining tree was constructed with a bootstrap of 1000 replicates. Genetic distances that are in the units of the number of base substitutions per site were computed using the Kimura-parameter method are given. The bar at the base of the tree indicates the scale for nucleotide substitutions per position. (B) Phylogenetic tree constructed for all identified HEV genotype 3 sequences. The analysis involved 34 nucleotide sequences, including 19 HEV strains isolated from domestic pigs in Vietnam. Bootstrap analysis values (percentages) are shown.