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. 2019 Jun 10;47(15):8207–8223. doi: 10.1093/nar/gkz503

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Mutational analysis of the spacer region. (A) Mutations introduced into the TMEV PRF signal in reporter plasmid pDluc. (B–E) RNAs containing the TMEV PRF signal were translated in WG (B and D) or RRL (C and E) with 1.8 μM recombinant TMEV 2A or with dialysis buffer (DB). Markers are in lane 1. The right-most lane in (B) and (C) was run on the same gel but a single intervening lane has been excised, as indicated by the vertical black line.