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. 2019 Jun 10;47(15):8207–8223. doi: 10.1093/nar/gkz503

Figure 6.

Figure 6.

Interchangeability of 2A proteins and lack of activity of L protein in PRF stimulation. (A) RNAs containing the EMCV or TMEV PRF signal were translated in RRL with 1.8 μM recombinant EMCV 2A or TMEV 2A or with dialysis buffer (DB). Markers are in the middle lane. (BC) RNAs containing the EMCV PRF signal were translated in WG (B) or RRL (C) with 1.6 μM recombinant EMCV 2A or TMEV 2A, and/or with EMCV L in varying amounts, or with dialysis buffer only (DB). Markers are in the first lane.