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. 2019 Jun 28;42(8):284–296. doi: 10.1097/CJI.0000000000000286

FIGURE 1.

FIGURE 1

Construction and expression of αFR-CARs in NK-92 cells. A, Schematic representation of αFR-CARs. All 3 generations of αFR-CAR contained a CD8α signal peptide, an anti-αFR scFv, an IgG1 Fc fragment, a CD8α hinge region, and a CD28 transmembrane (TM) region. Moreover, αFR-ζ contained a CD3ζ intracellular signaling domain, αFR-28ζ contained a composite CD28-CD3ζ intracellular signaling domain, and αFR-28BBζ contained a composite CD28-CD137-CD3ζ intracellular signaling domain. B, The relative expression levels of different αFR-CAR mRNAs normalized to GAPDH mRNA expression in NK-92 cells were detected by qPCR. The data are expressed as the means±SEM of triplicate samples. C, Surface expression of αFR-CARs was analyzed by flow cytometry. The filled green histograms indicate isotype control, whereas the filled red histograms indicate the αFR-CARs expression.