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. 2019 Jun 28;42(8):284–296. doi: 10.1097/CJI.0000000000000286

FIGURE 2.

FIGURE 2

Specific cytotoxicity of NK-92-αFR-CAR cells against αFR-positive cancer cells. A, Surface expression of αFR on the established human cancer cell lines was analyzed by flow cytometry. The filled green histograms indicate isotype control, whereas the filled red histograms indicate αFR expression. B, Surface expression of αFR, as measured by the change in the mean fluorescence intensity (MFI). C, Cell killing by NK-92 cells (filled circles), NK-92-EV cells (filled squares), NK-92-αFR-ζ cells (filled triangles), NK-92-αFR-28ζ cells (filled inverted triangles), and NK-92-αFR-28BBζ cells (filled rhombus) was investigated in the LDH cytotoxicity assay after coculture with target cells at the indicated E/T ratios. All data are expressed as the means±SEM of triplicate samples. Statistical analysis was shown for NK-92-αFR-28BBζ cells versus NK-92-αFR-ζ cells (the upper labels, represents significant difference) or NK-92-αFR-28ζ cells (the nether labels, *represents significant difference). ##P<0.01; **P<0.01; *P<0.05; ns, P≥0.05.