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. 2019 Jun 28;42(8):284–296. doi: 10.1097/CJI.0000000000000286

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Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/

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Antigen-specific expansion and antigen-induced apoptosis of NK-92-αFR-CAR cells. A, Expansion of the effector cells in the presence or absence of αFR protein using the CCK-8 assay, as described in the Materials and methods section. B, IL-2 release of the effector cells in the presence or absence of αFR protein using the ELISA assay, as described in the Materials and Methods section. C, Apoptosis of the effector cells induced by SK-OV-3 cells or A-431 cells was analyzed by flow cytometry. D, Apoptosis ratio of the effector cells induced by SK-OV-3 cells or A-431 cells. The E/T ratios are indicated in the Materials and Methods section. All the data are means±SEM of triplicate samples. Statistical analysis is shown for NK-92-αFR-28BBζ cells versus NK-92-αFR-ζ cells (represents significant difference) or NK-92-αFR-28ζ cells (*represents significant difference). ##P<0.01; **P<0.01; *P<0.05.