Figure 4.
MMS-induced stabilization of KKIP5 is independent of ATM and ATR kinases. (A) MMS-induced increase in KKIP5 protein level was not affected by depletion of ATM, ATR or both ATM and ATR. KKIP5 was endogenously tagged with a triple HA epitope, whereas ATM and ATR were each tagged with a PTP epitope. TbPSA6 served as a loading control. (B) MMS-induced increase in KKIP5-positive cells was not affected by depletion of ATM, ATR, or ATM and ATR. Two hundred cells were counted for each cell sample. Error bars indicate S.D. from three independent experiments. ns, no statistical significance.